PrimeView™ Human Genome U219 Array Strip

Catalog number: 901613

Description:

Proven performance from the industry standard
The Affymetrix™ Human Genome U219 Array Strip enables expression profiling of four samples at a time using probe sets with an emphasis on established, well-annotated content. Sequences used in the design of the array were selected from the UniGene database 219 (build date March 30, 2009), RefSeq version 36 (July 13, 2009), and full-length human mRNAs from GenBank™ (downloaded May 12, 2009).

The Affymetrix Human Genome U219 Array Strip enables you to:

• Measure gene expression of more than 36,000 transcripts and variants per sample
• Get accurate and reproducible gene expression data by using multiple independent measurements for each transcript

Key benefits

• Enables increased productivity and efficiency through parallel processing
  •   Process four samples on a single array strip
• Offers excellent gene expression accuracy and reproducibility
  •   Multiple independent measurements per transcript for increased confidence in your results–11 probes per set for well-annotated sequences, 9 probes per set for the remainder
• Provides complete coverage of the annotated genome
  •   Highest transcript coverage in the industry with most recent annotation–covers all well-annotated genes and transcripts from RefSeq

Content profile

• The Affymetrix Human Genome U219 Array Strip is comprised of more than 530,000 probes covering more than 36,000 transcripts and variants, which represent more than 20,000 genes mapped through UniGene or via RefSeq annotation
• The EST and mRNA sequences used in the design were clustered and assembled to create consensus sequences that represent alternative splice forms. Each assembly was then analyzed for orientation and alternative 3' end evidence
• Content was chosen to cover all well-annotated genes and transcripts from RefSeq v36 (NM_ accession type) and by leveraging all available EST and mRNA evidence that fall into the same clusters, to rigorously detect alternate 3' ends of those well-annotated genes
• More than 1,000 probe sets represent transcripts that have no official gene symbol in UniGene, but are based on predicted RefSeq sequences and UniGene clusters with good evidence of actual transcription

Specifications: